Multi-step starter and yeast generation count

[PierreLo]

Hi guys,

A quick question for you.
Let's assume that generation 0 is the initial generation of a fresh yeast package.
If I do a two-step starter and then harvest some yeast, is the yeast harvested generation 1 or generation 2 yeast?

Thanks,

PL

 

[marc1]

I would count it as 2 passages.
It's a vague, inexact concept, though.

If you did cell counts, you could find out how many population doublings they've gone through, which is a better guess at how many times the cells have divided.

But that's probably overkill for most homebrewing needs.