isomerization
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Never really thought about that. You got a point there. But yeah, I’ve been pulling it off the mash.
*Edit...I guess that’s the wrong answer. I have thought about it, briefly.....with everything like the stir bar and flask sanitized beforehand, I’ve always been willing to accept the wort being at 150 something was enough to kill anything that could outcompete the yeast being pitched into it.
Sorry, but I don’t think that’s right. Clearly if it’s worked for you without massive contamination, then the risk must be low, but below 160F is a danger zone for lots of spoilage bacteria.