quick question...about cell counting.

[DuncB]

I did get the idea of using monitor with notepad on screen as the background to view xray films if an xray light box wasn't available published in a medical journal.
Those were the days using films, nowadays its all digital.

 

[bracconiere]

The science dept is in the cellar... Apparently there's an entry fee.

damn it! i don't want to go down there with egor though!

 

[DuncB]

Not sure whether the haemocytometer would work vertical? There can't be much volume

 

[bracconiere]

Not sure whether the haemocytometer would work vertical? There can't be much volume

i layed my spare tv on it's back and used the scope on it....all i saw were the RGB lines from the screen....but it was promising, think the white light tracing thing will work way better then the light coming from the top....and YES @IslandLizard i'm about up to the cost of a digital $200 scope at this point! but having more fun doing it!

 

[DuncB]

CRT monitor would have that problem, white perspex / polythene in between screen and your slide?

 

[bracconiere]

CRT monitor would have that problem, white perspex / polythene in between screen and your slide?

it's a flat panel...but yeah, a piece of paper would be a good idea....and i don't sweating on it, or as you say persperex over it? LOL

but damn it, i SOOOO amm going to learn how to count! anyone know where to get hemocytometer covers for cheap? this one didn't come with any? or what they're called so i can search for them?

 

[IslandLizard]

and YES @IslandLizard i'm about up to the cost of a digital $200 scope at this point!

I don't think that would fit the purpose for you either. Chances are optical resolution is still poor, not getting better with typically (highly) inflated "electronic" magnification levels. Small crap in, bigger crap out.

IMO, the only solution is a truly optical microscope with an appropriate condensor light. I've seen them on auction sites dealing in surplus lab equipment, coming from labs and colleges. It may take a while to find the right piece for your needs, and possibly around the same $200, I dunno.

But always know what you're buying, and whether it suits your purpose while it fits your budget!

 

[bracconiere]

But always know what you're buying, and whether it suits your purpose while it fits your budget!

somebody asked how long yeast stays fresh in the fridge? i was like, hum, need to do a cell count, some way to figure out how many are alive or dead after a certain amount of time.... now i have a $15 digital scope, a vial of methylene blue...a hemocytometer..... but having a blast! they always get you with the cheapie at first!

 

[bracconiere]

around the same $200, I dunno.

yeah i can get an optical for $150 off ebay, with digital camera $200.... but to me this is a gag to see how i'd do a cell count if alcohol was illegal here....and i cared enough not just to buy fruit juice...

 

[passedpawn]

it's a flat panel...but yeah, a piece of paper would be a good idea....and i don't sweating on it, or as you say persperex over it? LOL

but damn it, i SOOOO amm going to learn how to count! anyone know where to get hemocytometer covers for cheap? this one didn't come with any? or what they're called so i can search for them?

This is where I bought mine (site used to be cynmar.com). I paid $1.35 for a box in 2012 (which will last you forever). They are now $1.68. Inflation!

https://yoursciencehub.com/microscope-cover-slips-square-22-x-22mm.html

 

[bracconiere]

This is where I bought mine (site used to be cynmar.com). I paid $1.35 for a box in 2012 (which will last you forever). They are now $1.68. Inflation!

https://yoursciencehub.com/microscope-cover-slips-square-22-x-22mm.html

thanks! needed the right terminology! "Microscope Cover Slip" i was searching for hemocytometer cover slips....

got this off ebay.

https://www.ebay.com/itm/121694330276?hash=item1c558c1da4:g:KC8AAOSwu4BVlac9

$4.99 with 'free' shipping ain't bad...