So one guy is talking about resurrecting one very old and certianly almost dead smack pack and the other guy is talking about maintaining his yeast bank and these two guys think they're arguing about the same thing? Did I miss anything?
it's all the same thing? I just made starters for 4 yeast packs that were best by Aug-Oct 2022. Turned out great, lots of yeast to harvest once decanted. The first one I made a 2nd 1l starter to test and it took off within hours so it was pretty viable, I'd say. I did do 1c of 1.020 wort to start then added 1l of 1.040 or so of wort to each pack, gives them a gentler wake up from the back of the fridge...Did I miss anything?
So out of curiosity, does "just" mean last week, last month, or back in March?I just made starters for 4 yeast backs that were best by Aug-Oct 2022
3 were 9 months expired and one was 7 months. They were kept in the fridge.starters for 4 yeast packs that were best by Aug-Oct 2022.
Are those starters on stir plates? That would be a much better/more efficient way to propagate the yeast. They need Oxygen.in the last 2 weeks I made 4 starters, decanted, doing another 4 starters starting today (with fresh packs)
So are you making S-n-S starters now?It is a plate, but last week I learned of this bit:
It is a plate, but last week I learned of this bit:
https://www.experimentalbrew.com/2020/12/09/shaken-not-stirred-the-stir-plate-myth-buster/
https://www.homebrewersassociation.org/forum/index.php?topic=24447.msg311550#msg311550
I’m actually surprised more people don’t say they use an oxygen wand for the starter.
[Raises hand too].I’m actually surprised more people don’t say they use an oxygen wand for the starter.
Give it another day or 2.UPDATE: I made an 1800ml starter 24 hours ago, and there is zero activity. Every time I shake it, there is absolutely no foam. Smells like wort, not like yeast and beer. I know it's relatively early, but I've never had no response from a starter after one day. I'm pessimistic. The question is, should I be? I'm having technical issues trying to add a photo, but trust me, it's a dark, lifeless flask that I think is cackling at me. Any thoughts?
Yeah, it's more of a swirl. And a very careful swirl at that.Give it another day or 2.
BTW, how do you "shake" an (erlenmeyer) flask? Especially an almost full 2 liter one? I can't even swirl one when it's that full.
I don't. Would a glass growler fit the bill?Do you happen to have gallon (3.5 or 4 liter) "wine" jug with a screw lid, or a well fitting stopper? That makes a decent s-n-s vessel.
Shake it to create lots of foam.
You can't do that with an erlenmeyer flask, and shouldn't, it's not safe, much too fragile.
Yeah, got a gallon one? Or perhaps even better, two 1/2 gallon ones?I don't. Would a glass growler fit the bill?
Ah, it looks like I only have one half-gallon growler. Back to swirling, I guess.Yeah, got a gallon one? Or perhaps even better, two 1/2 gallon ones?
Make sure to always release the stopper or lid somewhat after shaking, so there won't be any pressure built up when the yeast gets active. Or "tent" the opening with aluminum foil.
Keep good sanitation, you don't want to grow bugs.![]()
I'd pour half of the starter into that growler, splitting the starter. That will make swirling the flask much more efficient with much less risk of blow off once it takes off.Ah, it looks like I only have one half-gallon growler. Back to swirling, I guess.
I'll give it a shot!I'd pour half of the starter into that growler, splitting the starter. That will make swirling the flask much more efficient with much less risk of blow off once it takes off.
Before transferring, homogenize the starter in the flask by giving it a good stir, using a long sanitized spoon, or stirring stick, or so.
S-n-s the growler as if it owes you money with the screw cap on it. But let air get in there (while CO2 off gases) between shakes using the loosely crimped aluminum foil cap. Use good sanitation with all that.
I'd pour half of the starter into that growler, splitting the starter. That will make swirling the flask much more efficient with much less risk of blow off once it takes off.
Before transferring, homogenize the starter in the flask by giving it a good stir, using a long sanitized spoon, or stirring stick, or so.
S-n-s the growler as if it owes you money with the screw cap on it. But let air get in there (while CO2 off gases) between shakes using the loosely crimped aluminum foil cap. Use good sanitation with all that.
Since it's s-n-s, whenever you can, say 4-6 times a day. More won't hurt, but there are diminishing returns at some point. When shaking you want to create as much foam as you can. Each (tiny) foam bubble holds 21% Oxygen, surrounded by a film of suspended yeast cells.How often should I shake with the lid on?