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I began washing and harvesting my own yeast about a year ago. I got excited and harvested 4 jars from 4 batches in the first month, leaving me with 16 jars of 4 different yeasts. Since I usually brew twice per month, I had a ton of yeast just sitting in my fridge. After using some of this stored (aka old) yeast in beers that came out less than perfect, I began tossing it - hours of work, down the drain. Then I had an idea - why can't I just harvest clean yeast directly from my starter? After trying out a few techniques, here's the process that seems most efficient:
Step 1: Make a starter (3 days prior to brewing) that is .5 liter larger than you need for your beer. Since the majority of 5 gallon batches require no more than a 1 liter starter, a standard 2000 mL flask or even gallon growler will work great. In the photo below, I needed a 2 L starter for a 10 gallon batch, so I made 2.5 L.
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Step 2: A couple days after making your starter, sanitize a 500 mL (1 pint) mason jar using the sanitizer of your choice. I prefer Iodophor.
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Step 3: Fill the sanitized mason jar directly from the starter - if you use a stir plate everything will be in suspension, otherwise shake it up a bit prior to pouring.
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As you can see, I poured about 500 mL of wort/slurry from my starter into the mason jar. After just a few minutes, you can already see the creamy white and very clean yeast settling to the bottom of the jar.
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Step 4: Place the capped jar in the fridge over night to crash the yeast.
If you plan to use the yeast within a week or so, you can decant the "beer" and pitch directly from this jar. However, if you like to use different yeasts and won't be returning to your freshly harvested yeast for awhile, you will want to store it in a more hospitable environment. Steps 5 and 6 address how to do this.
Step 5: Fill a 250 mL (1/2 pint) mason jar halfway with tap water then microwave it (without the lid!) for 2 minutes to sterilize and de-oxygenate the water.
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Remove the very hot jar and put the lid on (using pot holders... it's HOT!), then shake it up to sanitize the lid with the boiling water. Let this sit over night to chill to room temp. I usually do this right after harvesting my yeast from the starter.
Step 6: The next day, decant about 80% of the beer off of the pre-harvested yeast in the larger mason jar, making sure to agitate to release the yeast from the bottom of the jar. Then simply pour the yeast directly into the previously boiled water in the smaller mason jar.
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Cap tightly and place in the fridge. Within a couple days you will have very clear water on top of a clean and compact yeast cake. When you want to use this yeast, make a starter as usual, decanting most of the water off the yeast, leaving just enough to help break up the cake.
Some of the benefits I see to this method include:
  1. The yeast is un-hopped and as clean as it's ever going to be. In fact, this is basically how White Labs and Wyeast grow their yeast.
  2. Since you're only making one jar of yeast, you won't have to store a ton of yeast (some people won't like this point).
  3. You don't have to go through the "washing" process, which is a pain in the arse if you ask me.
  4. You can brew any beer you want, even a barley wine or RIS, and still harvest yeast, as you're getting the yeast prior to it fermenting the beer you will ultimately brew. I guess you will need a pretty large flask, though.
I hope this helps. I've used the same strain multiple times very successfully. I'll never go back to washing yeast again.
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Cheers!
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According to the WhiteLabs website, harvesting your own yeast is encouraged for approx 10 generations. After that the yeast starts to mutate/degenerate.
There should be less yeast fatigue with the method described here since the yeast isn't exposed to long periods of Alcohol and Carbon Dioxide So maybe double that if you never actually harvest from washing or get any infections?
You can read the source for yourself here: http://www.whitelabs.com/faq/beer-professional
 
Back in Nov. 2012, @bierzwinski said "I brew two 12 gal batches at a time, therefore 4 carboys. Using this method, I have one left over to make more starters with."
How many more starters can you make from that one leftover? How many times can you repeat this process?
It's brilliant! I'm going to be brewing next week, so I'm definitely going to try this!!
 
Anyone following this thread as it seems to relate?
https://www.homebrewtalk.com/f163/cold-hard-truth-about-rinsing-yeast-boiled-water-451925/
 
Im just wondering do you think that the .5 liter extra starter that you make is equal to a vial of white labs?
 
Thanks Bru - I stay in Singapore and offerings by Wyeast and White Labs are sold by local LHBSs here for about USD 15 (yes, fifteen US dollars - no typos here). Your method really helps to keep the costs under control.
 
I have the same question about dry yeast. I was planning on doing this same method but then read this and wasn't sure how true it is. I guess it doesn't matter if it depletes the reserves as lumpher says as long as I make sure to aerate the wort enough right?
https://www.homebrewtalk.com/f163/splitting-dry-yeast-410419/#post5186568
 
An even better alternative is using isotonic solution (water and salt) instead of plain water. In isotonic solution the yeast can the stored for years at room temperature.
NaCl solution can be bought from drug stores for cheap or you can make it at home with water and 0.9% table salt, so 9 grams of salt for every litre of water.
 
Having followed the directions listed above using the sterile water from the second smaller jar and keeping this is the bottom of the fridge, would there be a safe guess as to how long this would be good for after the second washing?
 
I've been doing this a while , but am i the only one who uses beer bottles rather than jars? they are so much more convienient
 
Hello, This is a great read. As I am fairly new to home brewing, I have a couple of questions : Would this be considered a "neutral" yeast ? Yeast might have explode (as there are many available now) since this was posted, how do you go about having or making a specialty beer yeast ? Or do you simply not worry about it.
Thanks
 
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