I prepared my first batch of yeast for my yeast bank on monday evening. everything went well, no mishaps. i followed FlyGuy's instructions pretty much to the letter. the only place i varied is that instead of putting the glycerine in each vial before autoclaving in the pressure cooker, i put 50ml of glycerine in a beaker covered with foil and put that in the pressure cooker. after that was cool i decanted the beer off the yeast and then used a pipette and bulb to transfer 100ml of yeast slurry into the beaker, giving me a 33% glycerine solution. this afternoon when i got home i took the vials out of the fridge, and put them in the freezer. 6 hours later they're not frozen. the glycerine was separating while they were in the fridge, so i'd shake them every time i opened the fridge. is this something to be concerned about? i feel like i did a really good job of getting just yeast out of the starter. should i have included a little beer/water. so far they're not separating like they did in the fridge, but still definitely not frozen. the glycerine i used is NOW brand vegetable glycerine. i got it at a health foods store, it's 100% pure and is "a USP and food grade, all natural product derived from vegetable oil". someone please tell me to not freak out!