bv805
Member
Can you get the slant's pre-filled instead of 'rolling your own' ??
Yes you can Morebeer.com sells them but it kinda takes the fun out of the process. Also I think they are kinda pricey
Can you get the slant's pre-filled instead of 'rolling your own' ??
Below is the spreadsheet I use to track yeast slants and hop inventory. I also put labels on each slant. One on top of the lid with the strain number and one on the side with the inoculation date and generation number.
http://db.tt/ESAq5CY
Jason
Wondering why you don't use sterile wort when mixing your agar? Only DME. Any reason?
When you put the solution in the pressure canner and bring it up to 250 degrees, it gets sterilized. Therefore no point in using pre-sterile wort.
I got started with slanting yeasts about 6 weeks ago. I've used 2 slants so far and it has worked great. Here's the part of my brew blog where I documented what I did. I had to remake my slants after the first attempt when I put in too much agar and the slants were way too thick.
http://littleshedbrewery.wordpress.com/category/yeast-propagation/
You can't reuse the culture medium unless you were to sterilize it again and some are saying the agar doesn't reheat/melt well so that's out. Agar is super cheap tho and if your slant tubes or plates are autoclave-able you can sterilize them and make new plates.
Btw, I ran into a local guy who sterilized the medium along with a glass syringe and uses sterile plates with great results. Doesn't seem like it's harder to sterilize the slants as described here tho and seems far less likely to become contaminated that way.
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