Are there any larger autoclavable vials than in the op? I'd rather not have to do two starters to get a frozen vial ready to pitch.
Or would a well stirred starter on a stirplate with one of these vials allow for good propagation?
You would need a pretty huge vial of frozen culture to get by with just a single step. Based on some vaguely scientific experiments with a hemocytometer, I think just two steps is even pushing it.