Freezing test tubes of yeast

[waynemil]

I have harvested yeast and filled test tubes with 50% yeast 25% water and 25% glycercine. I want to freeze, but in the fridge I am getting 3 distinct layers. Even after I shake it, it separates pretty quickly. Has enough glycercine been absorbed into the yeast to go ahead and freeze even with these layers?

Thanks in advance.

 

[mredge73]

Sitting overnight should be long enough to absorb.
You can pour off the excess water if you like or just put in the freezer, up to you.

 

[Firestix]

This is the guide that I use. https://www.homebrewtalk.com/freezing-yeast.html
But I didn't use test tubes. I used breast milk freezer bags. They were cheap, already sterilized, made to be freezable, and are clearly marked with volume amounts.