Building from 3L starter

[orangemen5]

I built a 3l starter (1.037) from about 30ml of yeast. Its been sitting for a week. I might have under estimated my initial yeast count. I Prob should have stepped it up from a one liter. I'd like to step it up one more time to make sure I have enough yeast but how would I do that when I started with such a large starter to begin with.

 

[duboman]

Go to http://www.mrmalty.com, enter your numbers and select pitch from slurry and enter your initial information and it will help you figure it out.

 

[orangemen5]

So if my slurry doesn't have enough cells. I already built my initial starter from a 3L at 1.037. Could I just do another 1L with a higher OG say 1.050 and get a little more growth. My initial starters been sitting for a week since I haven't been able to brew. Would like to get more yeast growth. But not sure i can when my initial starter was so big.

 

[flars]

So if my slurry doesn't have enough cells. I already built my initial starter from a 3L at 1.037. Could I just do another 1L with a higher OG say 1.050 and get a little more growth. My initial starters been sitting for a week since I haven't been able to brew. Would like to get more yeast growth. But not sure i can when my initial starter was so big.

Try cold crashing your starter. Decant the wort and make a new starter of about 1.5 liters in size. A large wort volume, without a proportionate amount of yeasts cells, will have a less than optimal growth rate in a starter.
Link to another tool for making starters. http://yeastcalc.com/

 

[WoodlandBrew]

3L at 1.036 (9°P) is 270g of DME => 270 billion cells grown.

At a cell density of about 300 billion per liter things start to get a little crowded, so I wouldn't add all of the resulting yeast to a 1 liter starter.

How many cells are you trying to grow?

See here for details:
http://woodlandbrew.blogspot.com/2013/03/side-by-side-starters-4-of-4.html

 

[orangemen5]

According to yeast calc I need 277 billion. My starter grew to 296. So I should be good. I'm wondering if I'm losing cells from letting my starter sit for a week on top of the starter wort.

 

[WoodlandBrew]

My blog has a post that addresses that very question:
http://woodlandbrew.blogspot.com/2013/01/abv-effects-on-yeast.html