How long does a yeast starter take before it's ready? I start by adding some yeast cells to a test tube with wort in it and let that go for 24 hours. Next I add the contents of the test tube to a 1000ml flask of wort and place it on a stir plate for 48 hours. Does this sound like a good routine? Will this also work for larger starters or will it take longer for the starter to be ready? Whats the longest a started will last? for example, I planned on brewing on Saturday but something came up so I need to wait until the following weekend. Will the started still be usable? Thanks, Mike