Alpha amylase from barley

[Nooor]

Hi i malted barley then dried by heating at 50degree c. Now can i add it after grinding to corn as amylaze alpha for sugar conversion? Or i need to roast it first before grinding to higher temperature?

 

[Miraculix]

Hi i malted barley then dried by heating at 50degree c. Now can i add it after grinding to corn as amylaze alpha for sugar conversion? Or i need to roast it first before grinding to higher temperature?

If malted and dried correctly, the enzyme concentration is now at it's peak. Heating it only reduces it.

So yes, you could use it.

 

[IslandLizard]

Yes, you can add your malted barley to the cracked/milled corn to convert that too.
The amount of enzymes in your barley malt determine how much milled corn you can convert.

I'd start at 50/50 malt/corn to get a baseline.
Yet, you may be able to convert 2-3x the amount of raw corn per amount of malted barley. By weight of course, not volume.

Do realize that the corn must be fully pregelatinized (by boiling or cereal mash) before it's accessible to the enzymes for conversion.

 

[DuncB]

You can buy those enzymes off the shelf and others as well. That will be quite a sticky mash so use some rice or oat hulls and or glucanase or an appropriate rest.

 

[mac_1103]

You can buy those enzymes off the shelf and others as well.

Alpha amylase and glucoamylase are cheap and easy to find (but maybe not in Egypt?). Same goes for beta glucanase. Depending on what one is trying to do, those might be enough. OTOH, beta amylase is expensive and difficult to source, and limit dextrinase is basically unobtanium.