i made my first starter on tuesday (one vial of WLP001), and grew at 20C on a shaker/incubator for 24 hours. I counted the cells with a hemocytometer and only came up with 50 billion cells per liter (did a 1L volume). I expected this to be 2-4 fold higher.
Is this a normal cell density under these conditions?
Is this a normal cell density under these conditions?