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yeast starter questions

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i made my first starter on tuesday (one vial of WLP001), and grew at 20C on a shaker/incubator for 24 hours. I counted the cells with a hemocytometer and only came up with 50 billion cells per liter (did a 1L volume). I expected this to be 2-4 fold higher.

Is this a normal cell density under these conditions?
 
i made my first starter on tuesday (one vial of WLP001), and grew at 20C on a shaker/incubator for 24 hours. I counted the cells with a hemocytometer and only came up with 50 billion cells per liter (did a 1L volume). I expected this to be 2-4 fold higher.

Is this a normal cell density under these conditions?


How is it possible to pitch 100 billion cells into 1 liter of wort, ferment it on a stir plate at 68 degrees and end up with only 50 billion cells? :confused:

I would say this is not normal. What was the production date on the vial ?
 
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