Baron von BeeGee said:
Will you? I thought if you pitched the same volume/same gravity that there would be the proper # of yeast cells and they would just get to work without entering a growth phase. My assumption was that to 'step up' you would have to pitch a greater volume of wort than you had previously.
I agree with the Baron... from what I've read, you have to increase the amount of wort to "step-up" a starter... for example, one 50 ml vial stepped up to 500 ml for 24-48 hours, then stepped up again to 2000 ml for another 24-48 before pitching... (These are my steps when using a stirrer)
Decanting and replacing the wort with the same volume will not increase the available space for yeast to grow into, so once maximum density is reached for a given volume, all you are doing is fermenting the new wort... I guess you would get "some" growth if you are not using a magnetic stirrer, because you wouldn't be at true maximum density, and replacing the wort would add oxygen and sugar and re-suspend the yeast... but as I use a stirrer, the only way I can increase the number of yeast cells is to increase the wort volume...
The following table lists the starter size necessary to reach "optimum" cell count for each way of preparing a starter... (from Maltose Falcons article I posted above)... the only reason you would be using larger volumes is if you had stepped up the starter to increase the total cell count:
Table 5. Approximate Starter volumes to achieve 10 million cells/ml.
_______________________Concentration_______Starter Volume
Starter treatment ________(millions/ml) ________for 5 gallons
traditional:......................... 20........................ 10 quarts
shaken:............................. 60 ........................3.3 quarts
intermittent aeration:........... 92 ........................2.2 quarts
continuous stirring: .............180-360................. 0.75 quarts
So making a starter by pitching one vial of yeast in normally aerated wort and then waiting (no shaking or extra aeration involved) you would need 2.5 gallons to get the proper number of cells to pitch in a 5 gallon batch!!! (and this should be done in 3-5 steps to optimize growth patterns of the yeast) That's why pitching onto a yeast cake works so well, you are very close to optimum fermentation density (less what yeast didn't flocculate out/ got transfered to secondary etc.)
anyway, that's my $0.02 worth of it
mikey