Thanks for your responses. I've got 4 vials of White Labs 802 at 43% viability, and figure I can step it up 3x to reach my cell count for a brew on Sunday after next. For this brew, I'll use DME for my starter, and then use my next wort as a foundation for my next starter.
This is the procedure that I'm going to follow, in the future, for 10 gallon batches of lager*. It may seem a little OCD, but I find that it helps because I'm not an analytic thinker. Suggestions and corrections are appreciated.
Using Wort to Make a Starter
Step 1.
1. Plan for an extra gallon of wort in the batch.
2, As the boil is completing and you have cooled a sample and tested for OG, remove a gallon of wort (3785.41 mL) from the boil.
3. Use the formula to determine how much water to add to bring the SG down to 1.040*.
4. Mix the wort and water in a clean two-gallon container.
5. Place wort in 800 mL mason jars and pressure can them at 15 psi for 20 minutes.*
6. Let cool and label jars. Store in a cool, dark place until needed.
Step 2
1. Sterilize a 5L flask and foam stopper: put 2000 mL of water in the flask, put on a foam stopper, bring the water to a boil, and boil for at least 10 minutes.
3. Cool down the flask and remove the water.
4. Add wort to flask as determined by a yeast starter calculator.
5. Add yeast to flask and use stir plate to keep yeast in suspension.
6. After 24-48 hours, cold crash yeast for at least two days*.
7. Decant yeast and repeat above as necessary.
Notes:
1. I also plan to make some starter wort out of DME, and have it on hand, just in case I need to step up my yeast 3x instead of 2x.
2. I would use the formula provided above as follows, assuming an OG of 1.058:
(3785 + x) mL * 1.040 = 3785 mL * 1.058 + x mL * 1.000
Solve for x.
3936 + 1.040x = 4004 + x
-68+ 0.040x = 0
0.040x = 68
x = 2720
Adding 2720 mL of plain 1.000 water to 3785 mL of 1.058 wort, yields 6505 mL of 1.040 wort, or enough to fill 8 large mason jars.
3. Make sure the yeast has fallen to the bottom of the flask, and that there is a clear demarcation between the yeast and wort, before you decant. I find that this takes longer using a lager yeast.
4. If using DME, bring water in container to boil, reduce to simmer, and then add DME while stirring until it dissolves. If you add DME to cold water and then attempt to pressure can, it may clump up. Add hot wort with dissolved DME to mason jars, and can as per usual.
5. Pressure cookers have different designs. Most pressure cooker manufacturers instruct you to vent for 10 minutes before adding the weight (if you have an old weighted release valve). But my old Mirro pressure cooker has a release valve on the side, so the procedure is to add the weight from the beginning, turn the heat on high, and wait for the pressure to build. Once the weight starts rattling, reduce the heat so that it rattles intermittently, and start timing from that point.
6. 1.040 is the high end of an SG for a starter. It can be anywhere from 1.030 to 1.040. Higher than 1.040 and you run the risk of stressing the yeast.