I have duotights and evabarrier on both the gas and liquid sides of my kegerator, and also on my 5lb co2 tank. Probably was noted above, but when seating the tubing go slooooooow, and twist it a little bit until it pops in. Another small trick I use is holding back the little dohickey that you push in to release the tubing, while inserting it (I fortunatley have small fingers). It does sound like the one that won't seat could be defective.