Rinsed yeast CELL COUNTING / VIABILITY

[laserghost]

Using Mr. Malty's pitching rate calculator and calculating viability by date, I'm wondering if the decline of viability on the "Repitching from Slurry" tab is appropriate for rinsed yeast. Or would rinsed yeast have better viability than a straight up slurry. The rinsing definitely separates out a good bit to most of the trub, so should I expect better viability than what the "Repitching from Slurry" tab indicates?

Perhaps in between "Repitching" and the "Liquid Yeast" tab?

I'm using some rinsed yeast from back to September and November '12. I have 4 vials averaging maybe 35 mL of flocced yeast each for a 1.039 blonde ale.

According to Mr Malty's viability decline (saying 10% viability), I would want to pitch all 4 mason jars of slurry into a 1.5 L starter. Does that sound right or like too much yeast?

 

[mkravitz13]

Using Mr. Malty's pitching rate calculator and calculating viability by date, I'm wondering if the decline of viability on the "Repitching from Slurry" tab is appropriate for rinsed yeast. Or would rinsed yeast have better viability than a straight up slurry. The rinsing definitely separates out a good bit to most of the trub, so should I expect better viability than what the "Repitching from Slurry" tab indicates?

Perhaps in between "Repitching" and the "Liquid Yeast" tab?

I'm using some rinsed yeast from back to September and November '12. I have 4 vials averaging maybe 35 mL of flocced yeast each for a 1.039 blonde ale.

According to Mr Malty's viability decline (saying 10% viability), I would want to pitch all 4 mason jars of slurry into a 1.5 L starter. Does that sound right or like too much yeast?

this is sorta the same question that im wondering. how much is appropriate to pitch into a starter from a refrigerated yeast fraction?

 

[laserghost]

I've also started using this method where you make the starter .5 L bigger and pour that much off into a single mason jar to store, instead of rinsing from the trub.

That said, I have no problem pitching all 4 jars worth of slurry in if I have to (they are about 4 months old).