I brew two batches of high gravity lager about twice a year, so I have experience stepping up a single smack pack or vial of yeast for both batches.
I use a stir plate, a one-gallon pickle jar, and a couple of Mason jars. The following process takes about a week, so plan ahead.
So here's how my most recent double-brew yeast preparation went. Both recipes (Maibock & Vienna Lager) were scaled for 5.5 gallons with an OG of 1.070.
Homebrew Dad's online yeast calculator tells me I need a total of 533 billion yeast cells for 5.5 gallons of 1.070 beer, so now I know where I need to finish at.
Step 1: Make a 3 liter starter at 1.037 on a stir plate. Add the contents of your smack pack or vial. The yeast are usually done about 24 hours later. This gets me to 521 billion yeast cells.
Step 2: Cold crash the pickle jar for 24 hours.
Step 3: Pour off nearly all the beer. Then swirl until all of the yeast cake has been loosened from the bottom of the pickle jar. Pour this off into two mason jars. Put one of the mason jars in the refrigerator.
Step 4: Make another 3 liter starter at 1.037 on a stir plate. Add the contents of your mason jar. The yeast are usually done about 24 hours later. This gets me to 667 billion yeast cells in the pickle jar.
Step 5: Cold crash the pickle jar for 24 hours.
Step 6: Pour off nearly all the beer. Then swirl until all of the yeast cake has been loosened from the bottom of the pickle jar. Pour this off into a mason jars. Put the mason jar in the refrigerator.
Repeat steps 4, 5, and 6 for the second Mason jar from step 3, and you'll end up with two Mason jars with 667 billion yeast cells each, more than enough for my lager recipes.