eddiewould
Well-Known Member
Hey,
Wondering whether this would work (in theory at least):
Lets say you have a small yeast culture (e.g. 50ml) with unknown count & viability (and no way to measure).
If you were to make a starter with n steps (of the same size), would you approach a limit thereby you can know (with some certainty) how many cells you've got?
For example
1) Pitch unknown quantity into 1L starter (1.040)
2) Ferment out, crash cool, drain off liquid
3) Add another 1L of 1.040 wort (with nutrients)
Repeat steps #2 & #3 say n=5 times. Surely you'd get less growth with each iteration, eventually arriving at some limit representing the number of cells a 1L culture can sustain.
Wondering whether this would work (in theory at least):
Lets say you have a small yeast culture (e.g. 50ml) with unknown count & viability (and no way to measure).
If you were to make a starter with n steps (of the same size), would you approach a limit thereby you can know (with some certainty) how many cells you've got?
For example
1) Pitch unknown quantity into 1L starter (1.040)
2) Ferment out, crash cool, drain off liquid
3) Add another 1L of 1.040 wort (with nutrients)
Repeat steps #2 & #3 say n=5 times. Surely you'd get less growth with each iteration, eventually arriving at some limit representing the number of cells a 1L culture can sustain.