How many times does cultured yeast need to be stepped up to be pitchable?

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Paradigm

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My friend and I are going to be making a Double IPA this weekend hopefully using the heady yeast we gathered. We poured the last ~20% of 2 cans into our flask and added starter wort and left it at 68 for about a month (because we forgot about it, tbh).

After that we cold crashed and decanted and stepped it up by adding it to another 1.5L starter wort, which we've had on a sitrplate for about a week. We're going to step it up once more and use that for this Sunday.

I've been going under the rough assumption that the first step brings it to the equivalent of a smack pack in cell count. The second starter brings it to pitchable. The third step up makes it pitchable for an DIPA.

Thoughts?
 
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