I've just started working on building a yeast bank and I'm having a hard time approximating yeast cell counts.
I took a White Labs vial manuf 09 Jan 13 and made a 3.8L starter. Yeast Calc and Mr Malty tell me that I should have 375m cells afterwards. So I cold crashed and decanted, sworled the mixture like crazy to make it as unform as possible and poured into 8.25 40ml vials. 5 vials went into the fridge, and the other 3.25 went back in to be stepped up for next weekend's brew.
The problem is that I'm comparing them to the Wyeast graphic below and coming up with odd numbers. I should have about 45 billion cells in each vial, but my vials look closer to the middle one (87 billion per vial).
What am I doing wrong? There's no way the picture is correct and I actually made 718 billion cells.
I took a White Labs vial manuf 09 Jan 13 and made a 3.8L starter. Yeast Calc and Mr Malty tell me that I should have 375m cells afterwards. So I cold crashed and decanted, sworled the mixture like crazy to make it as unform as possible and poured into 8.25 40ml vials. 5 vials went into the fridge, and the other 3.25 went back in to be stepped up for next weekend's brew.
The problem is that I'm comparing them to the Wyeast graphic below and coming up with odd numbers. I should have about 45 billion cells in each vial, but my vials look closer to the middle one (87 billion per vial).
What am I doing wrong? There's no way the picture is correct and I actually made 718 billion cells.