I have a pretty god starter going from one bottle of DGA. I pitched the sediment I to a normal starter mixture (2 cups water, 1/2 cup DME, forgot yeast nutrient). The yeasts built up a little bit, but not a whole lot (less than a white labs vial). I added another starter mixture which had little effect. I decanted the mixture off the yeast and threw in some fresh starter mixture (3 cups of water this time, 1/2 cup DME, yeast nutrient) 2 days ago And have been keeping the temperature in the high 60s/low 70s for the starter and it has been going strong since then. This weekend I'll step up the starter again and hopeully it'll be ready for Monday's brew.
Also, I know this is an old thread, but I didn't want to create ANOTHER Pacman harvesting thread just to say I got some from 1 DGA bottle.