WoodlandBrew
Well-Known Member
I'm looking to explore some other methods of testing viability of yeast. Is any one familiar with the slide culture method? Or even better, have experience with the Yeast - 6 method as described by the American Society of Brewing Chemists? (Or if anyone has a copy of it that would be wonderful)
When preparing slides the agar seems to tare pretty easy, and also has bubbles in it. To make counting more consistent I have tried to prepare a hemocytometer with a culture, but the 0.1mm depth doesn't allow much space for the sample and the agar.
What type of slide should I use? Any tips for working with small amounts of agar or gelatin?
This link is the best I could dig up so far:
http://kim.bio.upenn.edu/~simola/kimlab/lab/yeast_slide_culture.html
Any better ones out there?
When preparing slides the agar seems to tare pretty easy, and also has bubbles in it. To make counting more consistent I have tried to prepare a hemocytometer with a culture, but the 0.1mm depth doesn't allow much space for the sample and the agar.
What type of slide should I use? Any tips for working with small amounts of agar or gelatin?
This link is the best I could dig up so far:
http://kim.bio.upenn.edu/~simola/kimlab/lab/yeast_slide_culture.html
Any better ones out there?