StMarcos
Well-Known Member
I was going to divide some smack-packs/vials into test tubes (autoclaved equipment), and then step up these tubes when I need them. I was thinking of dividing 4 or 5 ways, and then using 500mL as a first step up, and then going from there. How can I extract information from pitching calculators to figure out smaller amounts of yeast growth?
Should I divide more/less, and/or use larger/smaller first-step starters? I'll be using a stir-plate with an initial heavy blast of O2.
The goal is to have 100 billion cells, so I can go from there as I am used to doing.
I've been looking at the charts in 'Yeast' and the mrmalty calculator, and confusing myself. Any hints/suggestions? I would love to be able to count cells, but don't have a scope or haeceomter (sp?).
Cheers/Thanks
Should I divide more/less, and/or use larger/smaller first-step starters? I'll be using a stir-plate with an initial heavy blast of O2.
The goal is to have 100 billion cells, so I can go from there as I am used to doing.
I've been looking at the charts in 'Yeast' and the mrmalty calculator, and confusing myself. Any hints/suggestions? I would love to be able to count cells, but don't have a scope or haeceomter (sp?).
Cheers/Thanks