From Kristoffer re-posting my Milk the Funk post back on page 2:
I stepped them up from the small vials he sent going 50ml > 100ml > 250ml >1L. From there I harvested 2 - 500ml jars of each of the F1 and F1C4 variants.
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As far as fermentation goes, I noticed the same thing when fermenting the beers as I did when stepping up the samples and making my starters for this batch - the F1C4 variant seemed to lag about 12-16 hours or so behind the F1 variant in terms of showing signs of fermentation, but then it caught up pretty quickly, produced a larger krausen, and finished quickly and slightly lower than the F1 variant. For this split batch the F1 finished at 1.012 and the F1C4 finished at 1.010.
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From those first 2 - 500ml jars I wound up with I gave 1 to a friend and then used the other to make a 2L starter. I used 1L for a 3 gallon batch and then again harvested 2 - 500ml jars for future use.
I noticed signs of fermentation pretty quickly even at the smaller stages, and that was after my wife stuck the original package in the freezer for a good 12-18 hours :smack:
Right now I'm propping up samples of Kristoffer's Lachancea Fermentati strain which is a yeast that produces lactic acid as well as ethanol and CO2
I've got a sour blonde with apricots dry-hopped with Citra and Amarillo going with the F1 varaint of this, this weekend I'm brewing a NEPA using the F1 variant, and then I'm going to do a pale ale with the F1C4 shortly.