You don't want to pressurize and then remove the connection. You want constant pressure, so that when you pour a beer more gas can move in to offset the liquid you've lost.
Right now, my setup is barbed. I think people like threaded more because you can be assured of a tighter seal and there're no clamps to mess with. For me, barbs seem to work fine, but it's a subjective kind of thing.
As for the regulators, I have a single dual-gauge regulator with a 4-way manifold. In retrospect I think having separate regulators would have been a better road. If you have different beers that need different pressures to pour well, a dual regulator will help immensely.
It's really only a matter of time before I buy 4 secondary regulators for my 4-way manifold. It allows much more flexibility.
For instance, I had punch, homebrew, and yuengling.
Homebrew loved 12psi and poured great, but yuengling was all foam and happier at either 2-3psi or 20psi. The punch wasn't carbonated well enough unless it as at 20-25psi.
So a single regulator limits you in certain ways. I had to sit at 2-3psi while the kegerator was in use, or the yuengling would spout foam forever. If you can live with adjusting all your brews to one pressure, then you're fine. Otherwise, consider a dual for your sanyo.