1) Best practice is to not use an airlock on a starter. You are growing yeast not brewing beer and they need oxygen to do that-hence aerating, shaking, stir plate, etc.
2) Do not pitch what is in the airlock, just what is still in your flask/ bottle as what is in the airlock is not protected from contamination
So next time leave the flask open? I just built a stir plate to set it on would you recommend leaving it open for the whole 24hr process?