Making 1 Gallon Experiments- how to divide and save yeast?

[jasonclick]

So there's a recipe I want to play with and was going to do some 1 gallon batches. 1 gallon is not enough to stick a vial of WLP029 in and it would be pretty pricey to buy a vial for every 1 gallon experiment. What's the best way of dividing up the vial to be used on several 1 gallon batches over the next 3-4 months?

 

[kenny_d]

The issue with splitting one vial is that the overall health of the live yeast is going to deteriorate over time. I recommend making a small starter and splitting from there.

 

[jasonclick]

that's kinda what I was thinking. maybe make a 1 liter starter and divide up. I guess the real question is how to properly store for 3-4 months. I know it should be good for a month or so.

 

[Calichusetts]

Maybe then just wash the yeast post-fermentation or even repitch on the yeast cake. I do 1 gallon, and big beers usually require the entire vial. Even when you get above 1.060, if the yeast is a month or two old, you will need the whole thing.

Even a two week old vial going into a 1.080 wort will take more than half the vial. If you brew often or know you will use the yeast, starter and split is the way to go

 

[kenny_d]

that's kinda what I was thinking. maybe make a 1 liter starter and divide up. I guess the real question is how to properly store for 3-4 months. I know it should be good for a month or so.

I use mason jars. When transferring the starter mixture/beer to them just minimize splashing/oxygen exposure, seal it and then toss it in the fridge. Sanatize first of course!