Sure. You can take a sample after all is transferred to the kettle. You will need to do some math, cause You probably have 7 gallons, and are going to boil down to 5.5 concentrating and raising the gravity. Make sure you give it a good stir first, and let the sample cool to 68 degrees or whatever your hydrometer is calibrated at.
If its low, you can add a bit of extra light dme, to make it up, or boil down further and make a smaller final volume.
If its high, you can water it down and make a larger batch, or replace some with water. Or not worry about it.