How long can we save Ale yeast in the fridge.

[bracconiere]

One at a time.

https://www.daraz.pk/products/usb-1...a0e.searchlist.list.6.7cb516fa6nxdY7&search=1
is what brewing wisdom needs...seems pretty affordable....for the $15 it would fun to f'around and just have fun trying to do it!

and then just print something like this out...you'll be able to tell dead from living also....

at least i think that's how to do it.....

 

[BrewingWisdom]

https://www.daraz.pk/products/usb-1...a0e.searchlist.list.6.7cb516fa6nxdY7&search=1
is what brewing wisdom needs...seems pretty affordable....for the $15 it would fun to f'around and just have fun trying to do it!

and then just print something like this out...you'll be able to tell dead from living also....

View attachment 770527


at least i think that's how to do it.....

As a newbie I think I don't need this at this stage.
If you want brewing to stay with me please take things slowly

 

[bwible]

Better than an airtight container would be an open glass container like a jam jar, or a small conical plask with a bit of aluminium foil over the top. You can keep it in place with a rubber band if you like.

Or a beer bottle or a wine bottle with an airlock on it if you can get the right size drilled stopper

 

[bracconiere]

As a newbie I think I don't need this at this stage.
If you want brewing to stay with me please take things slowly

hey man, now that YOU say that to me....i'm going to see if there's a similar microscope on ebay...collect some yeast slurry after a week in the fridge or two...and post a screen shot of the blot....i'm trying to help you and you're inspiring me! (on a side note i found a great use for the salinity hydro, testing sodium hydrogen carbonate concentrations! love it! keep it coming! i just tried to do it by ph before, which really didn't tell me if there was any excess...

 

[BrewingWisdom]

hey man, now that YOU say that to me....i'm going to see if there's a similar microscope on ebay...collect some yeast slurry after a week in the fridge or two...and post a screen shot of the blot....i'm trying to help you and you're inspiring me! (on a side note i found a great use for the salinity hydro, testing sodium hydrogen carbonate concentrations! love it! keep it coming! i just tried to do it by ph before, which really didn't tell me if there was any excess...

Glad to you that
Inspiring you or inciting you for a rebellion over there

 

[bracconiere]

i just ordered this, we'll say i'll give you count of living dead cells for a week in the fridge, and a month.....

https://www.ebay.com/itm/3343239697...r+ghgT60I3LTxhUOMS7f2RURccaM|tkp:BFBMmrGOh6Rg
and this time, you mowed the lawn and i just had to edge it!

i'm no rebel... just try to be a free thinker!

 

[bracconiere]

excited to do my first super duper cell count, got the haemogrid thingy printed already! just a week of anxiously waiting for the scope!

(the lines are clearer in real life.....just add a known amount of water, count one of the tiny squares...multiply by number of blot...look at dead vs. living cells...

i'll help you be the best brewer ever! honestly, i never gave a f about this! but now that i know i can get a scope for $15, sounds like fun!)

 

[Alan Reginato]

As a newbie I think I don't need this at this stage.
If you want brewing to stay with me please take things slowly

Exactly! Thats my point of view! Sometimes I got misunderstood because of that. Keep things simple until you want it to be more complex.

Homebrew is basically a simple hobby.

If you want to have a microbiology lab in you house or like to play industrial process simulation, that's ok! And it's nice! You beer probably will be less prone to infections and other problems than regular homebrew.

There are plenty of interesting equipment and process, usually expensive, but, please, don't tell beginners that they can't make good beer unless they have this or do that.

 

[bracconiere]

And it's nice!

damn right! i'm already fantazing about using this scope for looking at myclium also! crystal structures......damn and only for $15!

 

[Alan Reginato]

damn right! i'm already fantazing about using this scope for looking at myclium also! crystal structures......damn and only for $15!

I have a small low cost microscope in home, and it doesn't work with slurry... I couldn't see anything useful. You have to use some colour marks too, for living cells, I don't remember the name, it's blue something.

 

[BrewingWisdom]

Exactly! Thats my point of view! Sometimes I got misunderstood because of that. Keep things simple until you want it to be more complex.

Homebrew is basically a simple hobby.

If you want to have a microbiology lab in you house or like to play industrial process simulation, that's ok! And it's nice! You beer probably will be less prone to infections and other problems than regular homebrew.

There are plenty of interesting equipment and process, usually expensive, but, please, don't tell beginners that they can't make good beer unless they have this or do that.

Not just in homebrewing but in everything newbie learners should take things slowly. And should follow one source of learning.
Quite often newbies quit when they are overwhelmed with a lot of info at the beginning.

 

[BrewingWisdom]

i just ordered this, we'll say i'll give you count of living dead cells for a week in the fridge, and a month.....

https://www.ebay.com/itm/3343239697...r+ghgT60I3LTxhUOMS7f2RURccaM|tkp:BFBMmrGOh6Rg
and this time, you mowed the lawn and i just had to edge it!

i'm no rebel... just try to be a free thinker!

The price for this thing is same on daraz and eBay?

 

[bracconiere]

Not just in homebrewing but in everything newbie learners should take things slowly. And should follow once source of learning.
Quite often newbies quit when they are overwhelmed with a lot of info at the beginning.

and us old timers been brewing for 20 years need to put a spark back into it!

and yeah there is the blue...

wait now i've gotta get out the pole saw!

https://www.ebay.com/itm/2245150430...9CNmvbB/BSZCSP9p3OwUHtImZFPz|tkp:BFBMupiSiqRg

but i'm hoping this thread end in % dead yeast cells sitting in the fridge for 'X' amount of days!

 

[bracconiere]

The price for this thing is same on daraz and eBay?

well i looked up the exchange rate and a Rupee is 195 to 1 USD? so yes? it cost me $16 after tax? and a actual optical scope is only $60 here with 640x magnification if this thing doesn't work....

 

[BrewingWisdom]

well i looked up the exchange rate and a Rupee is 195 to 1 USD? so yes? it cost me $16 after tax? and a actual optical scope is only $60 here with 640x magnification if this thing doesn't work....

that optical scope is expensive.

 

[bracconiere]

that optical scope is expensive.

i wouldn't call it expensive...just time to gather my thoughts and slow down....

(it's the $5's and $10's they slip in without you noticing that will kill you though)

just play it like this..

 

[Ozarks Mountain Brewery]

I've bought one or two trust me their junk, don't even bother

 

[bracconiere]

I've bought one or two trust me their junk, don't even bother

what about this...?
https://www.ebay.com/itm/154033694900?hash=item23dd1fa8b4:g:MKUAAOSwRTJfH0RX
because this is the way i'm feeling?

"TELMU Microscope is an ideal product to engage children in scientific Observation and Discovery." and hell if i'm old enough to drink? even more fun right?

 

[Ozarks Mountain Brewery]

what about this...?
https://www.ebay.com/itm/154033694900?hash=item23dd1fa8b4:g:MKUAAOSwRTJfH0RX
because this is the way i'm feeling?

"TELMU Microscope is an ideal product to engage children in scientific Observation and Discovery." and hell if i'm old enough to drink? even more fun right?

haven't tried one, could be interesting.
remember in most cases you get what you pay for with brand new china made products, good luck anyway

 

[lumbergh]

I just did my first starter to test out the stir plate I built. The yeast slurry has been in the fridge for almost a year. How do I tell that it's alive? It's been on the stir plate for almost 2 days. I used fermcap.

 

[Ozarks Mountain Brewery]

the best way I've found to test yeast is the tried and true bread yeast proofing, just a few bubbles tells you its good

 

[bracconiere]

just a few bubbles tells you its good

wait a second here, aren't we trying to spin the brewing wisdom around? i know i'm having fun with it... lol



and no @BrewingWisdom i'm just having fun, learning brewing from square one, but with the difficulty set to 11

 

[Oleson M.D.]

I have a 5th generation Wyeast London Ale. Been in the fridge for one year. Sitting under 10.5% ABV beer.
We were going to dump it, but it has no tell-tale signs of having gone bad.

 

[IslandLizard]

I have a 5th generation Wyeast London Ale. Been in the fridge for one year. Sitting under 10.5% ABV beer.
We were going to dump it, but it has no tell-tale signs of having gone bad.

Don't blame you.
A few days ago I dumped the content of a good dozen (small) 4 and 8 oz jars containing old saved yeast that had become obsolete. Most were ranched from fresh starters at the time, in 2019 (!), and although a few looked light, bright and clean and smelled fine, some were dark or gray, smelling like burnt rubber. Time flies...

Believe it or not, a few even had the dreaded "I" written on the labels. Maybe at the time I thought I'd use them for some experimental wild fermentations some day, (that never came).

 

[bracconiere]

well i got my $15 microscope....and i can say i see cells! they're clear so i need to wait on the methylene blue dye, but for $30 i'll be able to say for sure how long a yeast slurry stays good in the fridge and how viable it stays....i'm uploading a video of the thing with the cells....but the video is 245MB big and i've only got 700kb up speed.....well to hell with waiting for 45 minutes....

here are a couple snaps...

those might look like drops of water on the lense, but they are actually what i'm pretty sure are yeast cells floating around, because on live video, they are floating and moving... and i think that clump is what makes this a top fermenting yeast, another question that was asked.....

pretty cool gadget for $15....going to be fun when i get the methylene blue to dye this stuff! should have it monday!

here's a better view of a cell....

never would i have thought i'd be looking at yeast cells! thanks man!

 

[bracconiere]

so this grid is 0.1`mm squares...i know this kinda off topic, but kinda related to how to tell how long yeast is good in the fridge....

but the little white things are yeast cells right? and although this isn't really good enough for a cell count.....or maybe i just would never in a million years whip out a tally counter to do it......it would be good enough with some methylene blue to tell how viable it was with?

so if i did a smear like this with a slurry saved in the fridge for a month with a couple drops of methylene blue, and too many of those little whit specks turned blue, toss it and pitch fresh?

edit and @BrewingWisdom i'll toss this yeast in the fridge, and take another picture 1 week out with the dye to tell how many cells are alive, 2 weeks, and 3 weeks...for both our benchmarking uses....

 

[bracconiere]

so that shot cost me 3,000 rs...and here is the dye to tell which cells are alive or dead....

https://www.daraz.pk/products/-i221...a0e.searchlist.list.6.6567477dgj0Ssp&search=1
240 rs is way better then the $26 2oz cost me here!

 

[McMullan]

Viability staining with MB is not worth bothering with, unless done properly, which actually takes more effort than the simplest vitality test, which is required anyway, for a viability test using MB. The (live) cells need to be metabolically active and deflocculated for reliable MB staining and accurate cell counts. Directly assessing stored slurry is a waste of time. Quality of the data is going to be absolutely *****. Trypan Blue is better, but activating the cells and deflocculating them remains necessary. I'd add about 10-20g concentrated slurry to 500ml starter wort. Start MB staining and cell counting after about an hour and then repeat every 3-4 hours, to monitor yeast growth. If the culture hits exponential growth within 12-24 hours it's viable enough, when finished, to step up to about 2.5L. Unless the stored slurry is fresh (< 1 week from fermentation finishing*), make a starter regardless. Viability by itself isn't enough. A crap fermentation - promoting yeast stress and potential off flavours - is achievable by pitching viable cells with low vitality. It's still under pitching.

*Harvesting yeast from a fermentation that finished a week or two before harvesting is already a week or two old and probably benefits from a starter.

 

[bracconiere]

Directly assessing stored slurry is a waste of time.

"that's entertainment!"

edit: this is what i plan on doing? you're saying it doesn't work this way?

https://geneq.com/biotechnology/en/...ne-blue-to-determine-the-yeast-viability-9378

 

[McMullan]

"that's entertainment!"

I always aim to please But it's true in most cases. A sample of stored slurry, that probably doesn't truly represent the slurry overall, mixed with too much MB* and not observed soon enough under a microscope, is just going to get messy. Making a genuine vitality starter is so much easier and requires as little as a big jam jar and some nutritious be wort. Not much more complicated than making a cup of tea. Even for a queen on her jubilee

*About 0.1% MB mixed with an equal volume of slurry diluted enough to not overwhelm the field of view and be counted with some accuracy is essential. Repeat at least a few times to average out variable eyesight and ability to stay awake.