Help with yeast washing

[thelastchairbrew31]

Hey all.
Can someone help me identify the layers in this pic ? I am attempting to salvage a yeast sample from my recent brew. I am confused regarding what substance is the suspended yeast

 

[thelastchairbrew31]

Sorry about the sideways picture.

The top of my container is on the left with the foil. Sorry about that

G

 

[m00ps]

The middle is yeast The bottom is trub. Top is wort and the thin layer in between is slightly less flocculant yeast

Personally, I harvest yeast from the other end of the brewing process. I overbuild a starter and get clean, healthy, pure yeast that hasnt been exposed to hop compounds or had to go through an entire fermentation. Id suggest trying it out

 

[thelastchairbrew31]

Looks like this after 3 hrs in fridge
Same deal? Top two layers yeast?
Thanks again
G

 

[flars]

There is also a lot of yeast in the bottom layer with the hop debris.

 

[thelastchairbrew31]

Looks like this after 3 hrs in fridge
Same deal? Top two layers yeast?
Thanks again
G

 

[thelastchairbrew31]

The layer above the green layer has yeast in it?

 

[specharka]

It's easier to pour yeast off the trub before you decant it. Shake it up and wait ten minutes for the trub to fall out, then decant the rest of the "clean" yeast into a new vessel. You can repeat this process 2-3 times to get a very clean, creamy pitch of fresh yeast for your next batch.

 

[thelastchairbrew31]

Looks like this after 3 hrs in fridge
Same deal? Top two layers yeast?
Thanks again
G