First yeast freeze & is there an autoclave-able pipette or baster?

[displacedtexan]

Long story short, I am about to freeze my first yeast.

I streaked a plate (petri dish) with Pacman yeast straight from a smack pack last week. The plate has been at about 70 F ever since. I just placed a loop full of it in 20ml of 1.032 wort and am waiting for it to ferment out. After it ferments out, I plan on stepping it up to a 400 ml starter, than crash cooling it in the fridge and following the yeast bank wiki.

I plan on pouring most of the beer off the starter and then pouring the slurry into sterilized test tubes with about 1/3 glycerine.

Do other people just pour, or does there exist an autoclave-able pipette or baster?

Any other comments about my first freeze attempts?

Thanks!

 

[ArtVandelay]

I don't have yeast culturing experience but do have plenty of biology experience. Although pricey you could get an auto-pipet which has disposable plastic tips which can be autoclaved. Although this is probably overboard.

Something like this
https://www.usascientific.com/index.asp?PageAction=VIEWPROD&ProdID=2633

 

[weirdboy]

They make stainless steel turkey basters, although I'm not sure about the bulb.


You can also buy packs of disposable pipettes for dirt cheap.