That study illustrates two important points. First, that the standard international UV spectroscopy method for IBUs does not measure the actual concentration of iso-alpha acid, it measures the absorption at a specific wavelength of light of a group of compounds that are chemically similar to iso-alpha. Secondly, in the spectrophotometric method, compounds other than iso-alpha acids contribute to the measured IBU number — and this can yield similar IBU values in beers brewed with fresh or aged hops, despite differences in perceived bitterness. The other compounds that contribute to IBU in the spectrophotometric method include bitter oxidized beta acids, decomposition products of alpha and beta acids (which are not bitter) and some hop polyphenols (which are thought to be bitter), like xanthohumol.