2 liter starter

[jtrux]

Is the clumps at the bottom just flocked yeast? Last time I made a starter with wlp-940 I remember it being more powdered and whiter colored and less grey. Just want to make sure before pouring into my wort on Sunday. It’s at the bottom (not floating).

 

[jtrux]

Guess what I’m asking is does this look like infection.

 

[IslandLizard]

Is that the bottom of the flask you're showing?

Shouldn't there be a thick cake of settled out yeast on the bottom? Or did you swirl it up?
If so, it indeed looks like remaining caked on yeast after being swirled.
Before using, swirl some more to suspend all of it, don't leave any of that good stuff behind.
Are you planning to pour the whole thing without cold crashing and decanting?

For peace of mind, infections in a yeast culture don't settle out on the bottom like that, they tend to stay in suspension doing their job, multiplying in an effort for a complete takeover. Sometimes they form a pellicle on top, a white or light gray skin-like membrane, preventing other intruders from entering.

 

[jtrux]

Is that the bottom of the flask you're showing?

Shouldn't there be a thick cake of settled out yeast on the bottom? Or did you swirl it up?
If so, it indeed looks like remaining caked on yeast after being swirled.
Before using, swirl some more to suspend all of it, don't leave any of that good stuff behind.
Are you planning to pour the whole thing without cold crashing and decanting?

For peace of mind, infections in a yeast culture don't settle out on the bottom like that, they tend to stay in suspension doing their job, multiplying in an effort for a complete takeover. Sometimes they form a pellicle on top, a white or light gray skin-like membrane, preventing other intruders from entering.

Thank you. Yes it’s on the bottom after swirling. I swirled to re-suspend to pour off a small amount to a new container as starting material for a future batch (gen 3, this batch is gen 2). I am cold crashing now, will decant 80% of the liquid and re-suspend before adding to my wort. Thanks again, I just got concerned bc gen 1 (the first starter I inoculated with bought yeast), looked a little more powdery.

 

[IslandLizard]

Thank you. Yes it’s on the bottom after swirling. I swirled to re-suspend to pour off a small amount to a new container as starting material for a future batch (gen 3, this batch is gen 2). I am cold crashing now, will decant 80% of the liquid and re-suspend before adding to my wort. Thanks again, I just got concerned bc gen 1 (the first starter I inoculated with bought yeast), looked a little more powdery.

After decanting, swirl it up very well to get those sticky deposits off, they're good yeast!

I find tilting the flask back and forth works sometimes better than swirling when the deposits are in the middle. The slurry must wash them off the glass.

It's interesting you say that the first "from fresh" starter looked more powdery. I too have noticed subsequent starters to contain more stickier yeast. Proteins or another sticky substance floccing out, perhaps?

 

[Broothru]

After decanting, swirl it up very well to get those sticky deposits off, they're good yeast!

I find tilting the flask back and forth works sometimes better than swirling when the deposits are in the middle. The slurry must wash them off the glass.

It's interesting you say that the first "from fresh" starter looked more powdery. I too have noticed subsequent starters to contain more stickier yeast. Proteins or another sticky substance floccing out, perhaps?

Quite possibly a much higher density of yeast cells (as in billions more)?