OpenSights
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- Dec 9, 2017
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Thoughts? 18 minutes.
I'm looking into yeast banking using glycerin. Slanting is definitely a step above that.
when i grow shiitakes and oyster mushrooms, i use a lot steriler environment.....i have a glove box, big blower and HEPA filter....and i use petri dishes, and tape them shut with parafilm...
i'm not sure why he's sterilizing with the flame, but doing all this in open air....
Because a clean room and laminar flow hood cost money and space.i'm not sure why he's sterilizing with the flame, but doing all this in open air....
Now that’s way above my pay grade! But I understand about the sterile environment.
Because a clean room and laminar flow hood cost money and space.
Do you routinely test air quality in your "steriler" workspace to verify a particular ISO classification?
Sounds like a cool setup though, regardless.
Fair enough.
That really is cool.
Each brewing gadget I acquire has to be weighed against my wife killing me. When she ups my life insurance I know I've gone too far.
Fair enough.
That really is cool.
Each brewing gadget I acquire has to be weighed against my wife killing me. When she ups my life insurance I know I've gone too far.
i'm not sure why he's sterilizing with the flame, but doing all this in open air....
i'm not sure why he's sterilizing with the flame, but doing all this in open air....
He is not really working in the "open air". The flame produces an updraft of reasonably sterile air. I am not an expert on this, but this is the setup that I see advocated in most yeast culturing references. In some cases, he moves items out of the flame area towards the camera, which you would not do at home.
I have not figured out if I want to move into yeast banking. I have had good luck with harvesting yeast slurry and repitching that, but for that to work best you really need to repitch in a month (maybe 2) after harvesting. I enjoy getting several batches out of a $9 pack of yeast, but not sure the expensive of the equipment and the work to store and propagate a common yeast is worth saving $9.
Sterilizing the loop and flaming the tube opening are standard microbiological practice. As far as doing it in the open air, we did that in the micro lab all the time, and with a lot of moderately pathogenic organisms (Staph, Strep, Enteric organisms, etc).
This looks like a fairly standard, routine procedure that would pass "standard of care" practices in a hospital....
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