“Jason,
It is my suspicion that the overpitch achieves a lower gravity, as expected, because of the high numbers of cells available to do the work. The underpitch, however, eventually has a higher percentage of daughters vs older cells. The daughters have near 100% viability, high sterol levels and are raring to go, while older cells will have various viabilities, membrane condition, etc. Many of them may not be as ready for active fermentation as the daughters. I’d expect, therefore, for the underpitch to have a slower start than the overpitch or the control, but then to catch up pretty suddenly and attenuate well. Of course, all assumes relatively healthy yeasts, etc.”
I then asked if this is something they see in the brewery:
“I’d say yes, but only to a point. I think there is a curve to it. My gut feeling is that for a wort of average strength, you could cut the pitch rate from 30 – 50% and still achieve or exceed final attenuation vs a control. You’d have higher esters, but a revitalized (younger) yeast set afterwards. We will sometimes slightly underpitch when we want to put some more vigor back in a culture that otherwise seems healthy. Conversely, overpitching ages the culture – fewer daughters, over time, leaves you with a lot of battle-weary scarred cells with inflexible membranes that are no longer at their best. And not as many young, scar-free new cells.”