Change out the tubing, its less expensive than buying materials for each subsequent batch and not having it turn out as well as expected, disassemble anything else (i.e. spigots, siphon, soak everything that contacts wort post boil in hot water and PBW for a couple of hours. Check for scratches in your plastics, i.e. buckets, stir spoon, as those can hold bacteria. Heck if necesary replace the buckets, again not terribly expensive. Afte the PBW soak in Star San for a while. This is of course all contingent on making sure the procedures in your brewing haven't changed. Also, out of curiosity are you bottling with corn sugar? Do you boil it into a solution with water prior to adding to the bottling bucket? If not I dont if bacteria can harbor itself in the bottling sugars if it is being added dry into the beer prebottle. Just a thought, good luck.