I may never wash yeast again! - Home Brew Forums

Register Now!
Home Brew Forums > Home Brewing Beer > Fermentation & Yeast > I may never wash yeast again!

Reply
 
Thread Tools
Old 03-27-2012, 02:13 PM   #1
kh54s10
HBT_SUPPORTER.png
 
kh54s10's Avatar
Recipes 
 
Aug 2011
Tiverton, Rhode Island
Posts: 10,923
Liked 1668 Times on 1367 Posts



I have been freezing my liquid yeast.

This is even easier than washing yeast. Though it takes longer to get ready for brew day.

I start by making a starter larger than needed for my recipe.

Before pitching I make 4 vials to freeze. This is generation 1. I have 40ml vials and I add 10ml yeast, 10ml glycerin and 20ml water. Shake them and put in the refrigerator. When cool I put them in the freezer in a tub with some freeze packs. This is to counteract the defrost cycle of the freezer to keep the yeast frozen.

If I go the recommended 4 generations and make 4 new vials from each frozen vial I could do 256 brews. I obviously don't do that.

When planning a brew I start about a week early to propagate my yeast from a frozen vial. I make a small weak starter of about .25L. I run the stirplate about 18 hours. I then decant and feed another .5L starter. This gives me approximately the same amount of yeast as a vial or pack. I then use this to make a starter the size needed for my recipe.

I have frozen yeast from November 2011 and have recently used those successfully.
I also now have 6 varieties and 21 vials to use.

 
Reply With Quote
Old 03-27-2012, 02:21 PM   #2
dbrewski
 
dbrewski's Avatar
Recipes 
 
Oct 2011
Indeterminate
Posts: 1,011
Liked 52 Times on 46 Posts


Hey, whatever works. I tried to do the vial thing with gelatin and culturing, but it was a pain in the rear end. It just seems easier to have a couple of large jars of sterile water on hand if/when you want to wash the yeast. I had this vision of never buying yeast again, but truth is, eventually you will need to buy some more, either different types for new recipes or just to refill an often used type. Plus if you are like me, you run into certain situations, like near the end of viability of saved yeast, where you are starting to question whether or not it's propagating right, or too many generations, etc. So I just find being more casual about it, buying new or using washed, fits my style.

 
Reply With Quote
Old 03-27-2012, 02:24 PM   #3
sweetcell
HBT_SUPPORTER.png
 
sweetcell's Avatar
Recipes 
 
Jan 2012
Rockville, MD
Posts: 5,090
Liked 954 Times on 695 Posts


where does one buy glycerin? is it expensive?
__________________
What hops should I grow? Looking for cheap honey?
- Drinking: BPA made with ingredients from NHC, 2 blends of a rye sour: ECY20 + ECY34, local sour cherry kriek #2, brett'ed Belgian blond on raspberries
- Aging: Imperial Chocolate Milk Stout (half on coconut), sour blond on second-use cherries, English Barleywine (half on brett), 3726 saison w/ brett x2 (dregs mix & Lochristi), GNO 3724 saison w/ brett mix, sour cherry mead, acerglyn, and a few other sours...

 
Reply With Quote
Old 03-27-2012, 02:26 PM   #4
Revvy
Post Hoc Ergo Propter Hoc
HBT_LIFETIMESUPPORTER.png
 
Revvy's Avatar
Recipes 
 
Dec 2007
"Detroitish" Michigan
Posts: 40,921
Liked 3190 Times on 1885 Posts


Quote:
Originally Posted by sweetcell View Post
where does one buy glycerin? is it expensive?
Just about any drugstore. And, no....It's pretty cheap.



CVS/pharmacy
SKU #483707
6.0 OZ
0.42 LBS
$5.49
__________________
Like my snazzy new avatar? Get Sons of Zymurgy swag, here, and brew with the best.

Revvy's one of the cool reverends. He has a Harley and a t-shirt that says on the back "If you can read this, the bitch was Raptured. - Madman

I gotta tell ya, just between us girls, that Revvy is HOT. Very tall, gorgeous grey hair and a terrific smile. He's very good looking in person, with a charismatic personality... he drives like a ****ing maniac! - YooperBrew

 
Reply With Quote
Old 03-27-2012, 03:00 PM   #5
kh54s10
HBT_SUPPORTER.png
 
kh54s10's Avatar
Recipes 
 
Aug 2011
Tiverton, Rhode Island
Posts: 10,923
Liked 1668 Times on 1367 Posts


Quote:
Originally Posted by dbrewski View Post
Hey, whatever works. I tried to do the vial thing with gelatin and culturing, but it was a pain in the rear end. It just seems easier to have a couple of large jars of sterile water on hand if/when you want to wash the yeast. I had this vision of never buying yeast again, but truth is, eventually you will need to buy some more, either different types for new recipes or just to refill an often used type. Plus if you are like me, you run into certain situations, like near the end of viability of saved yeast, where you are starting to question whether or not it's propagating right, or too many generations, etc. So I just find being more casual about it, buying new or using washed, fits my style.
Yes, I will still have to buy new strains, and replace commonly used ones occasionally. But If I only use the 4 vials once that will save me abut $28 dollars over buying fresh.

When stepping up I can easily see if the yeast are propagating.

When I wash my yeast I cannot really tell how much of the slurry is yeast and how much is trub. So far mine have not stratified well into distinct layers.

I will on occasion pitch on a yeast cake. And [I]may[I] wash if needed but I love having varieties on hand and feel safer keeping the frozen yeast for a long time than keeping refrigerated, washed yeast.

 
Reply With Quote
Old 03-27-2012, 04:08 PM   #6
dbrewski
 
dbrewski's Avatar
Recipes 
 
Oct 2011
Indeterminate
Posts: 1,011
Liked 52 Times on 46 Posts


Quote:
Originally Posted by kh54s10 View Post
...And [I]may[I] wash if needed but I love having varieties on hand and feel safer keeping the frozen yeast for a long time than keeping refrigerated, washed yeast.
Good point, plus it sounds like you enjoy doing it, and that's what counts.

 
Reply With Quote
Old 03-27-2012, 05:17 PM   #7
Sherpa FE
The < 1% Club
HBT_LIFETIMESUPPORTER.png
 
Sherpa FE's Avatar
Recipes 
 
Oct 2007
Blairsville, Georgia
Posts: 815
Liked 18 Times on 14 Posts


Quote:
Originally Posted by Revvy View Post
Just about any drugstore. And, no....It's pretty cheap.



CVS/pharmacy
SKU #483707
6.0 OZ
0.42 LBS
$5.49
Florida must be the exception, I tried the following places; CVS, Walgreens, Wal-Mart pharmacy, Target Pharmacy, private pharmacy, and even feed stores.
NOTHING, NADA, ZIP, ZILCH,

I'm being told that it's becoming harder to buy, because Homeland Security has deemed it a "watched" item. Not sure about the validity of this statement.

actually made me give up on the idea of freezing yeast.

This thread is activating interest again though. (see what I did there?)
__________________
No matter how much money you THINK you are saving by brewing beer, you are still just pissing money away

 
Reply With Quote
Old 03-27-2012, 05:40 PM   #8
dragonbreath11
Recipes 
 
Apr 2011
Linden, New Jersey
Posts: 180
Liked 5 Times on 5 Posts


I also have done the glycerin thing. At times when making a starter from a fresh vial I will reserve a bit and put it directly into the glycerin/water solution so I know I have the freshest, cleanest, and uncontaminated yeast. I've revived frozen yeast after a year in the freezer and it started right up. Here's where I purchase my Glycerin.

http://www.mountainroseherbs.com/bul...isc.php#bu_gly



16oz for 8.75+ shipping. They also carry bitter orange peel, irish moss, and many other brewing spices and goodies if anyone cares.
Click image for larger version

Name:	glycerine.jpg
Views:	988
Size:	12.4 KB
ID:	54449  

 
Reply With Quote
Old 03-27-2012, 05:58 PM   #9
Sherpa FE
The < 1% Club
HBT_LIFETIMESUPPORTER.png
 
Sherpa FE's Avatar
Recipes 
 
Oct 2007
Blairsville, Georgia
Posts: 815
Liked 18 Times on 14 Posts


Quote:
Originally Posted by dragonbreath11 View Post
I also have done the glycerin thing. At times when making a starter from a fresh vial I will reserve a bit and put it directly into the glycerin/water solution so I know I have the freshest, cleanest, and uncontaminated yeast. I've revived frozen yeast after a year in the freezer and it started right up. Here's where I purchase my Glycerin.

http://www.mountainroseherbs.com/bul...isc.php#bu_gly



16oz for 8.75+ shipping. They also carry bitter orange peel, irish moss, and many other brewing spices and goodies if anyone cares.
MOST excellent! Thanks for posting the link
__________________
No matter how much money you THINK you are saving by brewing beer, you are still just pissing money away

 
Reply With Quote
Old 03-27-2012, 06:00 PM   #10
rhamilton
Recipes 
 
Sep 2011
Austin, Texas
Posts: 1,249
Liked 66 Times on 54 Posts


Yea -- washing was too much hassle and no real reward. Raw trub works for less than 6 months, I'll use glycerin for long-term freezer storage.....
__________________
On Deck: Cornucopia Oktoberfest
Primary: Centennial Blonde v2, Ed Wort's Kolsch
Secondary: none
Kegged: County Jail Pale Ale, AHS Anniv IPA, AHS Brooklyn Brown, Raspberry Wheat, Blood Orange Hefe, Ranger IPA clone (x2), Newcastle clone, AHS Irish Red, Centennial Blonde
Bottled: Session Series Belgian Saison, Apocalypso, Pecan Porter, DFH 90 Minute Clone, Apfelwein (x2), Wytchmaker Rye IPA Clone, Vienna/Simcoe SMaSH, Munich/Cascade SMaSH

 
Reply With Quote
Reply
Thread Tools


Similar Threads
Thread Thread Starter Forum Replies Last Post
When NOT to wash yeast? kickz28 Fermentation & Yeast 2 04-13-2011 01:16 PM
My first yeast wash Bitterbrush Fermentation & Yeast 1 02-01-2011 09:52 PM
Only one yeast wash?? josers Fermentation & Yeast 7 10-13-2010 03:14 PM
Can i wash yeast from a starter of High Fluccuating yeast? GLoBaLReBeL Fermentation & Yeast 4 06-03-2010 12:00 AM


Forum Jump