Washing Yeast

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Whelk

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I've just started washing a yeast cake for the first time, and I have a few questions I was hoping someone could answer. I've been following the washing directions here: http://www.wyeastlab.com/hbrew/hbyewash.htm
Anyway, here are the questions. First, about how long does it take for the viable yeast to separate? IE, should I be taking the thinner liquid on top of the trub as soon as it's visible, or should I wait longer? Second, if I do have to wait longer, should I be refrigerating the yeast as it separates? Third, and I guess this will be answered by question one really, can I leave the yeast to separate overnight? Thanks, let me know if I've made any horrible mistakes or anything. if anyone has pictures of the process step-by-step, that'd be a huge help.
 
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