I have access to a -80C fridge and was planning on starting a yeast bank. What I would do is upon opening a white labs tube or wyeast smack pack freeze down 20 vials of 1ml of yeast with an additional 300 ul glycerine. I would like to use the least amount of step ups to get to a final of 2L along with the least amount of use on a borrowed pressure cooker. What would be the easiest way to do this?