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Atticmonkey · 11-27-2009, 05:54 PM

I figure this question has been asked before but I did a search and couldn't find anything so I'll ask it again. Can you put a lambic blend on a slant like you do for normal yeast for saving to a yeast bank? Im assumeing that the blend will grow on malt agar but Im wondering if when I take a sample out to grow up to a starter if I will get everything I want or just get part of the blend and not all the differnt strains that make it a blend. Anybody ever had any experience with this?

**Saccharomyces** · 11-27-2009, 10:24 PM

I don't know anyone who has tried but I am preparing to do this, I will be making both slants of 7*P wort media and stabs of 2*P dextrose media and inoculating both. Both will be propagated up the same prior to pitching. We will see how it goes. Worst case, the beer will be really really sour from a high concentration of bacteria, which is just fine with me.

Bokonon · 11-27-2009, 10:37 PM

I'm not sure you will have much luck unless you isolate each yeast strain and each bacteria and store them individually. I've also heard that lacto and pedio are better cultured in liquid vs on a solid medium.

sw341034 · 11-27-2009, 10:44 PM

I would agree with bokonon. If you tried to culture a mix of things you would have a host of microorganisms fighting for the same resources and inevitably I would think some would out compete others. I would say isolate first then store.

Martin2D · 11-28-2009, 03:11 AM

Isolating each individual organism in a blend is nearly impossible for the lay person. I recently did this, culturing temptation. I did 2 on just 8% DME and agar and two on 8% DME, some dextrose, and agar. It'll probably be a while before I get results, but they are cultured.

**Saccharomyces** · 12-09-2009, 03:01 PM

I'm going to try isolate and store method, starting with WLP655 and Wyeast Roeselare blend... will update this thread with my progress.

Hermit · 12-09-2009, 04:19 PM

Quote:

Originally Posted by Martin2D

Isolating each individual organism in a blend is nearly impossible for the lay person. I recently did this, culturing temptation. I did 2 on just 8% DME and agar and two on 8% DME, some dextrose, and agar. It'll probably be a while before I get results, but they are cultured.

Just curious. It seems like it would be nice if the agar/culture liquified at around 90/100 F?

**Saccharomyces** · 12-09-2009, 05:07 PM

Quote:

Originally Posted by Hermit

Just curious. It seems like it would be nice if the agar/culture liquified at around 90/100 F?

Bacteria have no problem growing on YPD media plates.

Hermit · 12-09-2009, 05:09 PM

Quote:

Originally Posted by Saccharomyces

Bacteria have no problem growing on YPD media plates.

Actually, what I'm thinking is warm them up until the turn liquid for pitching a starter. Below 90/100 in the fridge they are solid.

Edcculus · 12-09-2009, 05:11 PM

would slanting, then building a starter from a lambic blend throw off the ratio of the original blend? Would it be enough to notice?