bbbrew
Well-Known Member
So I count 400 cells in my hemocytometer using the simple method 5 squares of the 1 x 1 x .10 mm volume. The yeast was diluted in a 100 : 3 dilution (multiplier of 33). And I measure 175 ml of pure yeast volume - assuming 100% viability.
Doing this math give me 115 billion cells (400 x 5 x 33 x 175 x 10000).
I pitched this in a 19.5 plato wort of 11 gallons ale. Mr Malty's calculator says I need 608 billion cells.
I've got 5 times less than what is needed but the fermentation starts within 12 hours going strong. And this is what I have been seeing the last (3) batches where the amount of yeast cell that I have are a factor of 2 to 5 times less than what we are told they should be.
The wort was o2'd with pure oxygen for about 2 minutes at a low pressure (don't have a gas flow meter on the bottle). And then again about 3 hours after initially pitching.
When you look at Mr. Malty's calculator, it says 150 ml of yeast will be sufficient. But my initial calculation is well under the # of cells needed.
Any comments or suggestions from others would be welcomed as I'm scratching my head.
Doing this math give me 115 billion cells (400 x 5 x 33 x 175 x 10000).
I pitched this in a 19.5 plato wort of 11 gallons ale. Mr Malty's calculator says I need 608 billion cells.
I've got 5 times less than what is needed but the fermentation starts within 12 hours going strong. And this is what I have been seeing the last (3) batches where the amount of yeast cell that I have are a factor of 2 to 5 times less than what we are told they should be.
The wort was o2'd with pure oxygen for about 2 minutes at a low pressure (don't have a gas flow meter on the bottle). And then again about 3 hours after initially pitching.
When you look at Mr. Malty's calculator, it says 150 ml of yeast will be sufficient. But my initial calculation is well under the # of cells needed.
Any comments or suggestions from others would be welcomed as I'm scratching my head.