The fact you didn't see anything when transferring doesn't necessarily mean that the infection occurred in the secondary vessel. Pellicles are formed when aerobic organisms collect at the surface when there is oxygen available in the headspace but the organism responsible could already have been there in primary but simply didn't have the time to form a pellicle or there was no oxygen in the primary's headspace. In practice this means you need to sanitize/replace any possible source of infection cold-side, regardless of at which step the infection became apparent.This is actually a glass carboy secondary that I've never used before (recent hand-me-down from a friend). I didn't see anything in primary when I transferred it. I have not disassembled my kettle valve yet, not a bad idea.