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Are you asking if you can keep it and re-use it?

I understand the rough rule of thumb is if it's still got the tint it's still effective.
 
For some reason I'm thinking it loses it's effectiveness after several hours. It does seem to lose it's tint overnight.

I found this on the net from Not Exactly Sterile:

Make only as much iodophor sanitizing solution as you need for each use. Iodine is volatile and will outgas from the solution with time, losing its sanitizing ability. You may have noticed that an iodine solution left in an open glass jar will lose its brown color. If you do have leftover solution, store it in a tightly sealed glass jar or a PET plastic soda bottle. Solution stored this way is stable for about a week. Do not store the solution in other types of plastics because they will either absorb the iodine fairly quickly or allow it to volatize because of their gas permeability, again causing a loss of sanitizing ability. Iodophors, like other sanitizers, are most effective when used on clean surfaces. Proteins and other organic substances will bind the iodine making it unavailable for sanitizing purposes. Sulfur-containing compounds in particular are efficient iodophor inactivators.
 

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