Proper yeast rehydration is one of the most important steps to help ensure a strong and healthy fermentation. Normal inoculation for wine active dried yeast is 2 lb/1000 gal (25 g/hL). (approx 1 gram per gallon of must) When added properly, a 2 lb/1000 gal (25 g/hL) (1 gram per gallon) addition of wine active dried yeast results in an initial cell concentration of 3-4 million viable cells per milliliter of must/juice. Under favorable conditions, the initial cell population may increase up to 100-150 million viable cells per milliliter of must/juice before growth stops and alcoholic fermentation begins.
This biomass increase is critical for healthy fermentations..