so I'm watching brew masters, and I have a couple questions:
part 2 of that, how do they carbonate? do they have like a 10K pound co2 tank on premises?
how do they sanitize huge 100BBL tanks? manually? or is automated?
do breweries typically always repitch yeast? whenever they need new yeast, obviously they don't buy little white labs vials. Do they have like a 10 gallon vessel full of yeast?
that's about it.
There are a few ways breweries carbonate beer, depending on the size of the system. One way is to partially carbonate the beer naturally. That is to seal the fermenter to keep the CO2 inside. Pressure is monitored so as not to exceed the tanks rating. The beer is then filtered and run into another pressurized tank.
Carbonation can also be added in-line as the beer is being transferred from one tank to another. Finally in the serving tank (if in a pub) CO2 can be added through a carbonating "stone" (nowadays mostly sintered stainless).
For a 10-20 barrel brewpub system an 800 lb Dewar is used to hold CO2. This is usually left in place and a truck will come a certain number of times a month to refill it. Depending on use, nitrogen is stored in 400 lb Dewars and are swapped out when low. There are usually back-ups of 50 lb tanks of both CO2 and Nitrogen around just in case. Also a couple tanks of Oxygen for in line aeration of the wort during transfer from the wort chiller to the fermenter.
It is a bit hard to physically enter a fermenter. There are lots of safety regulations to meet when it comes to confined space entry. As the tanks have held CO2 from fermentation, there is a risk of them not having enough Oxygen inside them to sustain life. The larger breweries do have Oxygen monitoring gear to make sure it is safe to enter a room.
Most of the brewing vessels are cleaned with a CIP, Clean In Place, system. There is a sprayball located on top of the vessel and cleaning chemicals and water are pumped through it. The chemical mixture is pumped with a fair amount of force to the top and down the sides of the tank. It is held there for a bit and then pumped back up. Usually an alkaline cleaner is used first. Mixed with HOT water it is run for about 30 minutes. This is follow by a warm water rinse. Then an acid cleaner is used. The tanks are left in an acid condition until they are needed again. Then they are rinsed and a sanitizer is run through them.
Some breweries do indeed have yeast labs. Smaller operations and pubs do not. Yeast can be obtained from White Labs or Wyeast or other yeast banks. Usually they do not get fresh yeast in pitchable quantities. They do get larger amount than are in a vial, but they do build them up to a pitchable amount. Start with a 1 gallon starter, build that up to 5 and then 10 and so on. The idea is to get enough clean slurry to pitch at roughly one pound of slurry per barrel of wort.
The yest is cropped,, or collected from each batch and reused over and over again. Most brewers can get 10 -15 generations from each pitch. AB does get fresh yeast sent out from St. Louis every 5 generations.
In one the pubs I worked at, we would store the yeast in a 10 gallon stainless pot. Just like a brewkettle. In another, we used a couple of 10 gallon cornie tanks. We would use a 4 gallon stainless milk pail to hold the yeast that we were going to pitch. We would put the pail on a scale and measure out the amount we needed. We also added a bit of Chlorine dioxide to each pitch to make sure the yeast was clean.
I hoped these answers helped a bit.