Daybis
Well-Known Member
This thread makes me want to start my own yeast lab. hehe
PassedPawn, would this microscope work ok?
Costco - Celestron Touch Screen LCD Deluxe Digital Microscope
Thanks for your input
passedpawn, if you're taking requests for your "to do" list, could you count the number of cells in a bottle conditioned beer - say a Bell's Amber or Pale Ale?
BTW, PassedPawn, if I buy a traditional microsope (as opposed to that LCD one I just asked you about) I was considering this as an add on.
All right. I'll be picking up a bells 2-hearted this wkend (just made a clone and want to do a comparison). I'll give it a good shake to get the yeast into suspension and do that. It only takes a minute (after I get the dilution right, but I suspect I won't have to do that with bottled beer). I'll probably mess around with video again and post some more of that.
I should have been more specific - how about just from the dregs of the bottle, as that is what I'd be trying to culture.
passedpawn, if you're taking requests for your "to do" list, could you count the number of cells in a bottle conditioned beer - say a Bell's Amber or Pale Ale?
OK, saved my dregs from a bottle of 2-hearted this wkend.
Amount of dregs: 24ml (355ml in a bottle)That sounds about right, too.
Cells per ml: 850,000
Total cells in dregs / bottle: 302 million
jmf143 said:Thanks for doing this passedpawn. I appreciate the work you did.
Is 302 million the total number of cells and not just the viable ones? You talked about getting stain so you could differentiate the cells - I'm assuming you haven't gotten it yet.
smokinghole said:What type of stain are you looking to get? I count yeast a lot for viability and I am in the process of switching from methylene blue to alkaline methylene violet. The dye used to make the latter is methylene violet 3RAX. I have only really found it a Sigma Aldrich and they require you be a company or something order. I also believe that the dye is mixed into a glycine buffered alkaline solution. I made a little order mix up last time I sent something to the purchasing department, and neglected to include the dry dye in my order list. The cool thing is with the 1gram of dry dye you get a TON of solution to use. You simply take .1gram into 1L and 100ml of that stock is used to make 1L of actual die. So as you can see for $25 1gram of dye goes a real long goddamn way when you're using 1ml per sample. Your other option would be to order 100ml worth I think from White Labs. It's expensive compared to buying the ingredients in terms of what you get but it'd sure be easier.
My school has a very high end scope that I just found out has a sweet ass camera attached. As soon as this semester is over (its finals week now) my prof will show me how to not screw it up since it's like $50k, and then they'll set me free to take pictures of whatever I want.
If you need any help or info let me know.
I was planning on buying methylene blue. Why are.you considering going to the violet stain.
What type of stain are you looking to get? I count yeast a lot for viability and I am in the process of switching from methylene blue to alkaline methylene violet. The dye used to make the latter is methylene violet 3RAX. I have only really found it a Sigma Aldrich and they require you be a company or something order. I also believe that the dye is mixed into a glycine buffered alkaline solution. I made a little order mix up last time I sent something to the purchasing department, and neglected to include the dry dye in my order list. The cool thing is with the 1gram of dry dye you get a TON of solution to use. You simply take .1gram into 1L and 100ml of that stock is used to make 1L of actual die. So as you can see for $25 1gram of dye goes a real long goddamn way when you're using 1ml per sample. Your other option would be to order 100ml worth I think from White Labs. It's expensive compared to buying the ingredients in terms of what you get but it'd sure be easier.
My school has a very high end scope that I just found out has a sweet ass camera attached. As soon as this semester is over (its finals week now) my prof will show me how to not screw it up since it's like $50k, and then they'll set me free to take pictures of whatever I want.
If you need any help or info let me know.
You sure it makes 1000ml and not 1000L?
http://www.whitelabs.com/beer/Alkaline_Methylene_Violet_Solution.pdf
Right 1g makes 1L of stock. Each 10ml of stock makes 100ml of dye. Sorry each 1g makes 10L of dye not 1000L. The stuff you're buying at White Labs is the diluted stock. Not the .1g diluted solution.
They should be great, that damn scope is like $30,000. I want to see if I can take some florescent pictures. I just need to get a florescent dye that will bind to certain proteins on the cell surface or interior. This scope has a florescence attachment that can take some really cool pictures.
I am looking at microscopes for this and wanted to see if anyone thinks this type of microscope would be sufficient to see yeast buds or even do cell counts for viability. It is a USB 40x to 800x but not oil immersion. I'm curious as to what kidn of quality I could expect.
Amazon.com: 800x 2mp 8-led Usb Digital Microscope with Handsfree Stand (Support Windows 7(support 32 Bit Only!)/vista/xp/2000/mac Os X 10.5 or Above): Camera & Photo
Thanks for any insight!
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