Original Gravity Changed? ?

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Tizzomes

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So I brewed a all grain pale ale on Fri and had a gravity question. After chilling the beer I poured a bit into a glass to take a reading. I got an O.G of 1.040 I was off by 10! So the next day just out if curiosity I took another reading from the glass I never cleaned out. I got a reading of 1.056 by the way I'm using a refractometer.Would the following day reading be the one to go by??
 

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