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I usually do it before so I can just fill a 100ml cylinder with wort that I'm racking as opposed to sticking a wine thief into the fermenter. They will both give you the same reading unless you're pitching a huge starter.
 
For the little wort you're using to take your hydro reading, it's just not worth the risk of contaminating the batch by pouring it back in (in case your hydrometer, test jar, or beer thief aren't sanitized properly).
 
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